Level 1
Techniques in Molecular Biology
Registrations Open !!
Basic knowledge of Cell Biology, Biomolecules
Registrations Open !!
Basic knowledge of Cell Biology, Biomolecules
Techniques in Molecular Biology is an introductory yet comprehensive course designed to familiarize learners with both classical and modern experimental techniques used in molecular biology laboratories. The course emphasizes the principles, applications, and significance of key techniques involved in DNA, RNA, and protein analysis. The syllabus is structured to be completed in a total duration of 6 hours, ensuring focused learning with conceptual clarity.
| Day | Topic | Sub-Topic No. | Sub-Topic Title | Detailed Contents |
| Day 1 | Basic Molecular Biology Techniques | 1.1 | Introduction to Electrophoresis | Principle, types (agarose & PAGE), applications in DNA and protein analysis |
| 1.2 | Laboratory Orientation | Basic setup and lab instruments, buffers, reagents, safety practices | ||
| Day 2 | Nucleic Acid Isolation Techniques | 2.1 | DNA Isolation | Principle, sources, basic steps and significance |
| 2.2 | RNA Isolation | Overview, importance, precautions against RNase | ||
| Day 3 | Classical Molecular Techniques | 3.1 | Blotting Techniques | Southern, Northern, Western blotting - principles and applications |
| 3.2 | Restriction Enzymes Types | Recognition sites, applications in cloning | ||
| Day 4 | Polymerase Chain Reaction | 4.1 | Polymerase Chain Reaction (PCR) | PCR Principle Components, steps, amplification concept (Conventional PCR) |
| 4.2 | Types of PCR | Real Time PCR (qPCR), RT-PCR (Reverse Transcription PCR) | ||
| Day 5 | Protein Analysis Technique | 5.1 | Protein Extraction | Basic extraction methods and buffers |
| 5.2 | Protein Analysis | SDS-PAGE, ELISA - principles and applications | ||
| Day 6 | Data Analysis & Interpretation of Molecular Biology Experiments (Reading PCR amplification bands/Protein Band/DNA Sequences) | |||
| Know your Mentor | Contact Number | Email Id | Teaching Experience (in Yrs.) |
| Prof. (Dr.) Gajendra B. Singh | 8392938532 | gajendra.singh@cumail.in | 20 Years |
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